Genotype-dependent mass somatic embryogenesis: a chance to recover extinct populations of Pulsatilla vulgaris Mill.

Abstract The paper presents a technique for micropropagation of endangered in Europe and extinct Poland Pulsatilla vulgaris ex situ conservation the genetic resources. Genotype-dependent induction somatic embryogenesis rooting was revealed series two experiments (I II) performed under same experimental conditions. Shoot tips seedlings were best explants both Murashige Skoog (MS) medium supplemented with 0.25 or 0.5 mg L −1 BAP suitable embryos (SE) adventitious shoots. Mass SE obtained experiment I after transfer on ½ MS (2% sucrose) + 0.45 B1 extending culture to 2–3 months without passages. Rooting shoots critical point. Out seven media used I, only two, hormone free 5 NAA 3.76 B2 resulted altogether 36.4% rooted In II, embryogenesis, acclimatization failed. Regenerated plantlets converted from acclimatized vitro Both genome size, determined by flow cytometry, diversity analyzed ISSR markers, confirmed compatibility regenerants P. initial commercial cultivar. Regenerants II differed genetically Propagated tissues/organs (SE, shoots) could be source material cryopreservation, artificial seed production and/or regenerated restoration populations. Key Message via organogenesis pasqueflower developed as tool species recovery. point is that genotype-dependent, which affects repeatability also imposes applying molecular techniques confirm fidelity other genotypes.